Fig. 2. Effects of Ang II on LC3-II expression assayed using western blot. LC3-II expression is increased by Ang II compared with the control group in time- and concentration-dependent manners (A). At 12 hours of incubation, Ang II increases LC3-II expression in a concentration-dependent manner after controlled by β-tubulin (B). Data on the densitometric analysis are presented as mean ± SD (n = 3; ^*P<0.05 and ^**P<0.01). Control (100%); compared to conditions without Ang II.